Nanospray-targeted products

Phoenix S&T-Nanospray and Microspray solutions to meet the most challenging needs in proteomic and metabolomic research

Phoenix S&T’s dual-column Nimbus (motorized) and pneu-Nimbus (pneumatic) sources increase productivity up to 100%. A 1D+ or 2D nanoLC pump is all is needed to duplicate the efficiency of you existing nanoLC-MS setup. Columns and emitters are thoroughly cleaned before each analysis to eliminate carry-overs. While one column is being washed and loaded, the other column is in front of the mass spectrometer doing analysis. There is no more down-time for the nanoLC-MS system. The column switching and sheath gas drying of the emitters are controlled by an on-board microcontroller without the need of an external computer and subsequent problem-prone integration with the data system. The mass spec’s data system controls everything related to the analysis, as it is normally done. It is set-and forget.

Our basic single-column source is easy to use, has added functionalities such as sheath-gas enabled for microspray applications, and can be upgraded to dual-column.
Our column heaters are custom-made for your columns so that they can be placed directly in front of the mass spec inlet. Even with such dedication to each researcher’s specific need, the heater and controller together are priced scarcely more than a single long column. Today our column heaters: the pencil heater, butterfly heater and jailbreak heater are the most widely used capillary column heaters in the world.

Our nanospray emitters PST-mTips, are made of a stainless metal and are tapered only on the outside., which makes the emitters robust, non-clogging. We manipulate the interfacial properties between the emitter channel and the buffer, be it aqueous or organic or a mixture of both, to optimize the cone-jet mode formation, which results in the best ionization and vaporization conditions for the analytes in the buffer. This improvement also facilitates negative ion spray without the interference of corona discharge. The PST-mTip-25 has a 25 um i.d. and 360 um i.d. It is best for nanospray but also can be used for up to 10 uL/min flow rates. It can be used with the can be fitted to a fused silica capillary column without fittings and with only a Teflon ® sleeve.

For analytes that are not amenable for a metal emitter, we offer the polypropylene PST-noz emitter which has many of the properties of the mTips, but is primarily optimized for sub-microliter/minute flowrates. Indeed the same manipulation of interfacial property of the polypropylene nozzle is what enables the superb spray performance of the nozzle emitter. The nozzle emitter is indispensable for spraying analytes in complex matrices such as serum and plasma, or unprocessed plant extracts or fermentation broths. They are virtually impossible to blog!